script ii reverse transcription kit Search Results


rt pcr kit  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd rt pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt pcr kit/product/PCR Biosystems Ltd
Average 94 stars, based on 1 article reviews
rt pcr kit - by Bioz Stars, 2026-03
94/100 stars
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superscript ii reverse transcription kit  (Thermo Fisher)
90
Thermo Fisher superscript ii reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Superscript Ii Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript ii reverse transcription kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
superscript ii reverse transcription kit - by Bioz Stars, 2026-03
90/100 stars
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improm-ii reverse transcription kit with a random hexamer primer  (Promega)
90
Promega improm-ii reverse transcription kit with a random hexamer primer
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Improm Ii Reverse Transcription Kit With A Random Hexamer Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/improm-ii reverse transcription kit with a random hexamer primer/product/Promega
Average 90 stars, based on 1 article reviews
improm-ii reverse transcription kit with a random hexamer primer - by Bioz Stars, 2026-03
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excelrt™ reverse transcription kit ii  (SMOBIO Technology)
90
SMOBIO Technology excelrt™ reverse transcription kit ii
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Excelrt™ Reverse Transcription Kit Ii, supplied by SMOBIO Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/excelrt™ reverse transcription kit ii/product/SMOBIO Technology
Average 90 stars, based on 1 article reviews
excelrt™ reverse transcription kit ii - by Bioz Stars, 2026-03
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reverse transcription kit trans script one-step gdna removal and cdna synthesis super mix  (Beyotime)
90
Beyotime reverse transcription kit trans script one-step gdna removal and cdna synthesis super mix
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Reverse Transcription Kit Trans Script One Step Gdna Removal And Cdna Synthesis Super Mix, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reverse transcription kit trans script one-step gdna removal and cdna synthesis super mix/product/Beyotime
Average 90 stars, based on 1 article reviews
reverse transcription kit trans script one-step gdna removal and cdna synthesis super mix - by Bioz Stars, 2026-03
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prime script tm rt reverse transcription kit  (Genzyme)
90
Genzyme prime script tm rt reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Prime Script Tm Rt Reverse Transcription Kit, supplied by Genzyme, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prime script tm rt reverse transcription kit/product/Genzyme
Average 90 stars, based on 1 article reviews
prime script tm rt reverse transcription kit - by Bioz Stars, 2026-03
90/100 stars
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rnaus script reverse transcription kit  (LeGene Biosciences)
90
LeGene Biosciences rnaus script reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Rnaus Script Reverse Transcription Kit, supplied by LeGene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnaus script reverse transcription kit/product/LeGene Biosciences
Average 90 stars, based on 1 article reviews
rnaus script reverse transcription kit - by Bioz Stars, 2026-03
90/100 stars
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script™ reverse transcription kit  (Ribobio co)
90
Ribobio co script™ reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Script™ Reverse Transcription Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/script™ reverse transcription kit/product/Ribobio co
Average 90 stars, based on 1 article reviews
script™ reverse transcription kit - by Bioz Stars, 2026-03
90/100 stars
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primescript™ rt reagent kit impromm-ii reverse transcription system  (Promega)
90
Promega primescript™ rt reagent kit impromm-ii reverse transcription system
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Primescript™ Rt Reagent Kit Impromm Ii Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primescript™ rt reagent kit impromm-ii reverse transcription system/product/Promega
Average 90 stars, based on 1 article reviews
primescript™ rt reagent kit impromm-ii reverse transcription system - by Bioz Stars, 2026-03
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4xezscript reverse transcription mix ii (with gdna remover) kit  (EZBioscience)
90
EZBioscience 4xezscript reverse transcription mix ii (with gdna remover) kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
4xezscript Reverse Transcription Mix Ii (With Gdna Remover) Kit, supplied by EZBioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4xezscript reverse transcription mix ii (with gdna remover) kit/product/EZBioscience
Average 90 stars, based on 1 article reviews
4xezscript reverse transcription mix ii (with gdna remover) kit - by Bioz Stars, 2026-03
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miscript reverse transcription kit ii #218160  (Qiagen)
90
Qiagen miscript reverse transcription kit ii #218160
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Miscript Reverse Transcription Kit Ii #218160, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miscript reverse transcription kit ii #218160/product/Qiagen
Average 90 stars, based on 1 article reviews
miscript reverse transcription kit ii #218160 - by Bioz Stars, 2026-03
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fastgenescriptase ii reverse–transcription pcr kit  (Nippon Genetics)
90
Nippon Genetics fastgenescriptase ii reverse–transcription pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Fastgenescriptase Ii Reverse–Transcription Pcr Kit, supplied by Nippon Genetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fastgenescriptase ii reverse–transcription pcr kit/product/Nippon Genetics
Average 90 stars, based on 1 article reviews
fastgenescriptase ii reverse–transcription pcr kit - by Bioz Stars, 2026-03
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Image Search Results


(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Journal: PLOS Pathogens

Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi

doi: 10.1371/journal.ppat.1013849

Figure Lengend Snippet: (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Article Snippet: All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures.

Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Tandem Mass Spectroscopy